|Title:||Professor, Bioinorganic Chemistry - Department Chair|
|Office:||Department of Chemistry 1068 W. Sheridan Rd Chicago, IL 60626|
|Phone:||Phone: (773) 508-7045 or (773)508-3100|
B.Sc. with First Class Honors, 1979, Imperial College, University of London, UK
Ph.D., 1984, University of California, Los Angeles
My main area of interest deals with the application of multinuclear NMR and fluorescence techniques to the investigation of the biochemical basis of manic-depression and hypertension. In addition to developing spectroscopic methods for monitoring metal ion transport and binding, we have applied multinuclear NMR and fluorescence spectroscopy to the investigation of the abnormal transport and binding properties of Li+, Na+, and Mg2+ ions in red blood cells, neuroblastoma cells, and purified G (guanine-binding) proteins. Multinuclear NMR and fluorescence methods provide non-invasive tools for monitoring metal ion transport in biological tissues. Moreover, they also provide new information at a molecular level concerning binding of metal ions to cell membranes and intracellular components. This information is essential for obtaining an understanding of the roles of metal ions in biological fluids in healthy and disease states.
Other areas of research interest include the biophysical investigation of abnormal transport of Cl- in cystic fibrosis, the biological and toxicological functions of vanadate, vanadyl, and cesium ions, as well as structural-functional relationship studies of copper, zinc superoxide dismutase.
Mota de Freitas, D., Castro, M.M.C.A., and Geraldes, C.F.G.C.: Is Competition Between Li+ and Mg2+ the Underlying Theme in the Proposed Mechanisms for the Pharmacological Action of Lithium Salts in Bipolar Disorder? Acc. Chem. Res. 2006, 39, 283-291
Layden. B.T., Abukhdeir, A.M., Malarkey, C.M., Oriti, L.A., Salah, W., Stigler, C., Geraldes, G.F.G.C., and Mota de Freitas, D.: Identification of Li+ Binding Sites and the Effect of Li+ Treatment on Phospholipid Composition in Human Neuroblastoma Cells: A 7Li and 31P NMR Study. Biochim. Biophys. Acta 2005, 1741, 339-349.
Montezinho, L.P., Duarte, C.B., Fonseca, C.P., Glinka, Y., Layden, B., Mota de Freitas, D., Geraldes, C.F.G.C., and Castro, M.M.C.A.: Intracellular Lithium and Cyclic AMP Levels are Mutually Regulated in Neuronal Cells. J. Neurochem. 2004, 90, 920-930.
Srinivasan, C., Toon, J., Amari, L., Abukhdeir, A.M., Hamm, H., Geraldes, C.F.G.C., Ho, Y., and Mota de Freitas, D.: Competition between Lithium and Magnesium Ions for the G-protein Transducin in the Guanosine 5'-Diphosphate Bound Conformation. J. Inorg. Biochem . 2004, 98, 691-701.
Layden, B.T., Minadeo, N., Suhy, J., Abukhdeir, A.M., Metreger, T., Foley, K., Borge, G., Crayton, J.W., Bryant, F.B., and Mota de Freitas, D.: Biochemical and psychiatric predictors of Li+ response and toxicity in Li+-treated bipolar patients. Bipolar Disord. 2004, 6, 53-61.
Layden B.T., Abukhdeir A.M., Williams N., Fonseca C.P., Carroll L., Castro M.M.C.A., Geraldes C.F.G.C., Bryant F.B., Mota de Freitas D.: Effects of Li+ transport and Li+ immobilization on Li+ / Mg2+ competition in cells: Implications for bipolar disorder. Biochem. Pharmacol. 2003, 66, 1915-1924.
Minadeo, N., Layden, B.T., Suhy, J., Metreger, T., Foley, K., Abukhdeir, A.M., Borge, G., Crayton, J., Bryant, F.B., and Mota de Freitas, D.: Testing Competing Path Models Linking the Biochemical Variables in Red Blood Cells from Li+-Treated Bipolar Patients Bipolar Disord. 2003, 5, 320-329.
Diven, C.F.; Wang, F.; Abukhdeir, A.M.; Salah, W.; Layden, B.T.; Geraldes, C.F.G.C.; and Mota de Freitas, D.: Evaluation of [Co(Gly)3]- as 35Cl- NMR Shift Reagent for Cellular Studies. Inorg. Chem. 2003, 42, 2774-2782.
Abukhdeir, A.M.; Layden, B.T.; Minadeo, N.; Bryant, F.B.; Stubbs, Jr., E.B.; Mota de Freitas, D.: Effect of Chronic Li+ Treatment on Free Intracellular Mg2+ in Human Neuroblastoma SH-SY5Y Cells. Bipolar Disord., 2003, 5, 6-13.