Richard C. Holz
| Richard C. Holz | ||
|---|---|---|
 |
Title: | Professor & Chair |
| Office: | FH 125 | |
| Phone: | (773) 508-7045 | |
| E-mail: | rholz1@luc.edu | |
Personal Information
NIH Postdoctoral Research Fellow,
Ph.D. The
M.S. University of Minnesota-Duluth, Chemistry, 1986.
AWARDS AND HONORS
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Invited speaker at the International Conference on Bioinorganic Chemistry, July 2009
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Invited speaker at the 237th American Chemical Society National Meeting, March 2009
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Invited speaker at the 11th Nano Science and Technology Institute (NSTI)-Nanotech-08 Conference June, 2008
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Invited speaker at the 235th American Chemical Society National Meeting, March 2008
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Reviewer for the Center for Research and Education on Environmental Implications of Nanotechnology Program, NSF, Jan. 2008
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Reviewer for the Collaborative Research in Chemistry Program, NSF, Nov. 2006
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Reviewer for the Chemical Instrumentation Program, NSF, April 2005
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Symposium Chair/Organizer, 59th Northwest Regional American Chemical Society Meeting, June 2004.
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Institute for Nanotechnology and Department of Chemistry Visiting Research Professor, Northwestern University, 2002-03
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Invited speaker at the Inaugural Gordon Research Conference on Metals in Medicine, July 2002
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Ad-hoc Reviewer for Physical Biochemistry Study Section, NIH, March 2002
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Symposium Chair/Organizer, Metals in Medicine 222nd ACS National Meeting, August 2001
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Utah State University, College of Science Researcher of the Year, 2001
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Board of Directors, Stokes Nature Center, Logan UT, 2001 to 2004
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Ad-hoc Reviewer for the Physical Biochemistry Study Section, NIH, June 2000
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Reviewer for the Chemical Instrumentation Program, NSF, May 2000
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Invited speaker at the “International Conference on Bioinorganic Chemistry”, July 1999
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Ad-hoc Reviewer for the Physical Biochemistry Study Section, NIH, June 1999
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Eccles Foundation Research Award, 1998
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Invited speaker at the Gordon Research Conference on Metals in Biology, January 1998
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Eccles Foundation Research Award, 1996
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Invited speaker at the 51st American Chemical Society Northwest Regional Meeting, June 1996
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Invited speaker at the 209th American Chemical Society National Meeting, April 1995
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National Institutes of Health Postdoctoral Research Fellow 1990-92
RESEARCH INTERESTS
The Holz research group interfaces the general areas of mechanistic enzymology and biophysical chemistry but recently, our interests have also moved into biomolecular nanotechnology. In general the Holz group is interested in structure/function studies of metalloenzymes, which includes the design, synthesis, and characterization of small molecules that function as potent inhibitors. Within these studies, we use a wide variety of biophysical methods such as enzyme kinetics, site-directed mutagenesis, isothermal titration calorimetry, UV-Vis, NMR and EPR spectroscopies. We have integrated our work on metalloenzymes with biomaterials by investigating nano to micro-sized biologically powered motors as well as biomolecule recognition at surfaces. Devises of this type have many potential applications but clearly offer new technologies for the design of molecular detection devices.
RECENT PUBLICATIONS (76 TOTAL)
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Mitra, Sanghamitra; Bennett*, Brian; Holz*, Richard C. "Mutation of H63 and its Catalytic Affect on the Methionine Aminopeptidase from Escherichia coli" Biochim. Biophys. Acta, 2009, 1794, 137-143.
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Gilner, Danuta M.; Bienvenue, David; Nocek, Boguslaw P.; Joachimiak, Andrzej; Zachary, Vincentos; Bennett*, Brian; Holz*, Richard C. "The dapE-encoded N-succinyl-L,L-Diaminopimelic Acid Desuccinylase from Haemophilus influenzae Contains two Active Site Histidine Residues" J. Biol. Inorg. Chem., 2009, 14, 1-10.
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Mitra, Sanghamitra; Job, Kathleen, M; Bennett*, Brian; Holz*, Richard C "Dissecting the Catalytic Role of D97 in the Methionyl Aminopeptidase from Escherichia coli" 6248-6259.
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Rao, Saroja; Holz*, Richard C. "Analyzing the Catalytic Mechanism of the Fe-Type Nitrile Hydratase from Comamonas testosteroni Ni1" Biochemistry, 2008, 47, 12057–12064.
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Waterson, Sarah; Mitra, Sanghamitra; Swierczek,
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Munih, Petra; Moulin, Aaron; Stamper, Carin; Bennett, Brian; Holz*, Richard C.; Ringe*, Dagmar; Petsko*, Gregory "X-ray Crystallographic Characterization of the Co(II)-substituted Tris-bound forms of the Aminopeptidase from Aeromonas proteolytica" J. Inorg. Biochem., 2007, 101, 1099-1107.
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Nieman, J. Karl C.; Holz, Richard C.; Sims, Ronald C. "13C NMR Analysis of Biologically Produced Pyrene Residues By Mycobacterium Sp. KMS in the Presence of Humic Acid" Environ. Sci. Technol., 2007, 41, 242-249.
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Mitra, Sanghamitra; Holz*, Richard C. "Unraveling the Catalytic Mechanism of Nitrile Hydratases" J. Biol. Chem., 2007, 282, 7397-7404.
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McGregor, Wade; Bennett, Brian; Holz*, Richard C. "Kinetic and Spectroscopic Characterization of the Manganese(II)-Loaded argE-Encoded N-Acetyl-L-Ornithine Deacetylase from Escherichia coli" J. Biol. Inorg. Chem., 2007, 12, 603-613.
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Davis, Ryan; Bienvenue, David; Swierczek, Sabina I.; Gilner, Danuta M.; Rajagopal, Lakshman; Holz*, Richard C. "Kinetic and Spectroscopic Characterization of the E134 Altered dapE-encoded N-succinyl-L,LDiaminopimelic Acid Desuccinylase from Haemophilus influenzae" J. Biol. Inorg. Chem., 2006, 11, 206-216.
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Desmarais, William; Bienvenue, L. David; Bzymek, Krzysztof P.; Holz*, Richard C.; Petsko*, A, Gregory; Ringe*, Dagmar "The High Resolution and Low pH Crystal Structures of the Aminopeptidase from Aeromonas proteolytica" J. Biol. Inorg. Chem., 2006, 11, 398-408.
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Mitra, Sanghamitra; Jiracek, Jiri; Zertova, Miroslava; Zakova, Lenka;Holz*, Richard C "A New Colorometric Assay for Methionyl Aminopeptidases: Examination of the Binding of a New Class of Pseudopeptide Analog Inhibitors" Anal. Biochem., 2006, 357, 43-49.
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Rozhok, Sergey; Fan, Zhifang; Nyamjav, Dorjderem; Liu, Chang; Mirkin, Chad A.; Holz*, Richard C. "Attachment of Motile Bacterial Cells in a Pre-Aligned Holed Microarrays" Langmuir., 2006, 22, 11251-11254.
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Frottin, Frédéric; Martinez, Aude; Peynot, Philippe; Mitra, Sanghamitra; Holz*, Richard C; Giglione, Carmela; Meinnel*, Thierry "The Proteomics of N-Terminal Methionine Cleavage" Mol. Cell. Proteomics, 2006, 5, 2336-2349.
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Copik, Alicja J.; Nocek, Boguslaw; Swierczek, Sabina I.; Ruebush, Shane; SeBok, Jang; D'souza, Ventris M.; Peters*, John; Bennett, Brian*; Holz*, Richard C "EPR and X-ray Crystallographic Characterization of the Product Bound Form of the Mn(II)-Loaded Methionyl Aminopeptidase from Pyrococcus furiosus" Biochemistry, 2005, 44, 121-129.
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D'souza, Ventris M.; Brown, Robert S.; Bennett*, Brian; Holz, * Richard C. "Charaterization of the Active site and Insight into the Binding Mode of the Anti-Angiogenesis Agent Fumagillin to the Mn(II)-Loaded Methionyl Aminopeptidase from Escherichia coli" J. Biol. Inorg. Chem., 2005, 10, 41-50.
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Copik, Alicja J.; Watterson, Sarah; Swierczek, Sabina I.; Bennett*, Brian; Holz*, Richard C. "Both Nucleophile and Substrate Bind to the Catalytic Fe(II) Center in the Type-II Methionyl Aminopeptidase from Pyrococcus furiosus" Inorg. Chem., 2005, 44, 1160-1162.
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Rozhok, Sergey; Kwang-Fu Shen, Clifton; Littler, Pey-Lih Ho; Fan, Zhifang; Liu, Chang; Mirkin*, Chad A.; Holz*, Richard C. "Methods for Fabricating Microarrays of Motile Bacteria" Small, 2005, 1, 445-451.
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Rozhok, Sergey; Holz*, Richard C. "Electrochemical Adhesion of Motile Bacteria to Gold" Tantala, 2005, 66, 538-542.
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Swierczek, Krzysztof; Copik, Alicja J.; Swierczek, Sabina I.; Holz*, Richard C. "Molecular Discrimination of ype-I Methionyl Aminopeptidases Over Type-II" Biochemistry, 2005, 44, 12049-12056.
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Bzymek, Krzysztof P.; Moulin, Aaron; Swierczek, Sabina I.; Bennett*, Brian; Ringe*, Dagmar; Petsko*, Gregory; Holz*, Richard C. "Kinetic, Spectroscopic, and X-ray Crystallographic Characterization of the Functional E151H Aminopeptidase from Aeromonas Proteolytica" Biochemistry, 2005, 44, 12030-12040.
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McGregor, Wade; Swierczek, Sabina I.; Bennett, Brian*; Holz*, Richard C. "The argE-Encoded N-Acetyl-LOrnithine Deacetylase from Escherichia coli Contains a Dinuclear Metallo-Active Site" J. Am. Chem. Soc., 2005, 127, 14100-14107.
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Bzymek, Krzysztof P.; Swierczek, Sabina I.; Bennett*, Brian; Holz*, Richard C. "Spectroscopic Characterization of the E151D and E151A Altered Aminopeptidases from Aeromonas Proteolytica" Inorg.Chem., 2005, 44, 8574-8580.
CURRENT FUNDING
National Science Foundation - (CHE-0652981) 03/1/06 -02/28/09 "Mechanistic Studies on Dinuclear Metallohydrolases that are Novel Antibacterial Targets" The long range goals of this research are to elucidate the roles of each metal ion is catalysis for the aminopeptidase from Aeromonas proteolytica and the dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase from Haemophilus influenzae (dapE). Using a mechanism based approach a new class of antibiotics that target dapE will be designed and synthesized.