Holz, Richard

	Title:	Professor & Associate Dean Resources and Planning
	Office:	FH 420
	Phone:	(773) 508-3092
	E-mail:	rholz1@luc.edu

Personal Information

NIH Postdoctoral Research Fellow, University of Minnesota, 1989 to 1992.
Ph.D. The Pennsylvania State University, Chemistry, 1989.
M.S. University of Minnesota-Duluth, Chemistry, 1986.
B.S. Bemidji State University, Major: Chemistry, Minors: Biology and Mathematics, 1984.

AWARDS AND HONORS

Invited speaker at the International Conference on Bioinorganic Chemistry, July 2009
Invited speaker at the 237th American Chemical Society National Meeting, March 2009
Invited speaker at the 11^th Nano Science and Technology Institute (NSTI)-Nanotech-08 Conference June, 2008
Invited speaker at the 235th American Chemical Society National Meeting, March 2008
Reviewer for the Center for Research and Education on Environmental Implications of Nanotechnology Program, NSF, Jan. 2008
Reviewer for the Collaborative Research in Chemistry Program, NSF, Nov. 2006
Reviewer for the Chemical Instrumentation Program, NSF, April 2005
Symposium Chair/Organizer, 59^th Northwest Regional American Chemical Society Meeting, June 2004.
Institute for Nanotechnology and Department of Chemistry Visiting Research Professor, Northwestern University, 2002-03
Invited speaker at the Inaugural Gordon Research Conference on Metals in Medicine, July 2002
Ad-hoc Reviewer for Physical Biochemistry Study Section, NIH, March 2002
Symposium Chair/Organizer, Metals in Medicine 222^nd ACS National Meeting, August 2001
Utah State University, College of Science Researcher of the Year, 2001
Board of Directors, Stokes Nature Center, Logan UT, 2001 to 2004
Ad-hoc Reviewer for the Physical Biochemistry Study Section, NIH, June 2000
Reviewer for the Chemical Instrumentation Program, NSF, May 2000
Invited speaker at the “International Conference on Bioinorganic Chemistry”, July 1999
Ad-hoc Reviewer for the Physical Biochemistry Study Section, NIH, June 1999
Eccles Foundation Research Award, 1998
Invited speaker at the Gordon Research Conference on Metals in Biology, January 1998
Eccles Foundation Research Award, 1996
Invited speaker at the 51st American Chemical Society Northwest Regional Meeting, June 1996
Invited speaker at the 209th American Chemical Society National Meeting, April 1995
National Institutes of Health Postdoctoral Research Fellow 1990-92

RESEARCH INTERESTS

The Holz research group interfaces the general areas of mechanistic enzymology and biophysical chemistry but recently, our interests have also moved into biomolecular nanotechnology. In general the Holz group is interested in structure/function studies of metalloenzymes, which includes the design, synthesis, and characterization of small molecules that function as potent inhibitors. Within these studies, we use a wide variety of biophysical methods such as enzyme kinetics, site-directed mutagenesis, isothermal titration calorimetry, UV-Vis, NMR and EPR spectroscopies. We have integrated our work on metalloenzymes with biomaterials by investigating nano to micro-sized biologically powered motors as well as biomolecule recognition at surfaces. Devises of this type have many potential applications but clearly offer new technologies for the design of molecular detection devices.

RECENT PUBLICATIONS (76 TOTAL)

Mitra, Sanghamitra; Bennett*, Brian; Holz*, Richard C. "Mutation of H63 and its Catalytic Affect on the Methionine Aminopeptidase from Escherichia coli" Biochim. Biophys. Acta, 2009, 1794, 137-143.
Gilner, Danuta M.; Bienvenue, David; Nocek, Boguslaw P.; Joachimiak, Andrzej; Zachary, Vincentos; Bennett*, Brian; Holz*, Richard C. "The dapE-encoded N-succinyl-L,L-Diaminopimelic Acid Desuccinylase from Haemophilus influenzae Contains two Active Site Histidine Residues" J. Biol. Inorg. Chem., 2009, 14, 1-10.
Mitra, Sanghamitra; Job, Kathleen, M; Bennett*, Brian; Holz*, Richard C "Dissecting the Catalytic Role of D97 in the Methionyl Aminopeptidase from Escherichia coli" 6248-6259.

Rao, Saroja; Holz*, Richard C. "Analyzing the Catalytic Mechanism of the Fe-Type Nitrile Hydratase from Comamonas testosteroni Ni1" Biochemistry, 2008, 47, 12057–12064.
Waterson, Sarah; Mitra, Sanghamitra; Swierczek, Sabina I.; Meng, Lu; Holz*, Richard C. "Kinetic and Spectroscopic Analysis of the Catalytic Role of H79 in the Methionyl Aminopeptidase from Escherichia coli" Biochemistry, 2008, 47, 11885-11893.
Munih, Petra; Moulin, Aaron; Stamper, Carin; Bennett, Brian; Holz*, Richard C.; Ringe*, Dagmar; Petsko*, Gregory "X-ray Crystallographic Characterization of the Co(II)-substituted Tris-bound forms of the Aminopeptidase from Aeromonas proteolytica" J. Inorg. Biochem., 2007, 101, 1099-1107.
Nieman, J. Karl C.; Holz, Richard C.; Sims, Ronald C. "13C NMR Analysis of Biologically Produced Pyrene Residues By Mycobacterium Sp. KMS in the Presence of Humic Acid" Environ. Sci. Technol., 2007, 41, 242-249.
Mitra, Sanghamitra; Holz*, Richard C. "Unraveling the Catalytic Mechanism of Nitrile Hydratases" J. Biol. Chem., 2007, 282, 7397-7404.
McGregor, Wade; Bennett, Brian; Holz*, Richard C. "Kinetic and Spectroscopic Characterization of the Manganese(II)-Loaded argE -Encoded N -Acetyl-L-Ornithine Deacetylase from Escherichia coli" J. Biol. Inorg. Chem., 2007, 12, 603-613.
Davis, Ryan; Bienvenue, David; Swierczek, Sabina I.; Gilner, Danuta M.; Rajagopal, Lakshman; Holz*, Richard C. "Kinetic and Spectroscopic Characterization of the E134 Altered dapE -encoded N-succinyl-L,LDiaminopimelic Acid Desuccinylase from Haemophilus influenzae" J. Biol. Inorg. Chem., 2006, 11, 206-216.
Desmarais, William; Bienvenue, L. David; Bzymek, Krzysztof P.; Holz*, Richard C.; Petsko*, A, Gregory; Ringe*, Dagmar "The High Resolution and Low pH Crystal Structures of the Aminopeptidase from Aeromonas proteolytica" J. Biol. Inorg. Chem., 2006, 11, 398-408.
Mitra, Sanghamitra; Jiracek, Jiri; Zertova, Miroslava; Zakova, Lenka;Holz*, Richard C "A New Colorometric Assay for Methionyl Aminopeptidases: Examination of the Binding of a New Class of Pseudopeptide Analog Inhibitors" Anal. Biochem., 2006, 357, 43-49.
Rozhok, Sergey; Fan, Zhifang; Nyamjav, Dorjderem; Liu, Chang; Mirkin, Chad A.; Holz*, Richard C. "Attachment of Motile Bacterial Cells in a Pre-Aligned Holed Microarrays" Langmuir., 2006, 22, 11251-11254.
Frottin, Frédéric; Martinez, Aude; Peynot, Philippe; Mitra, Sanghamitra; Holz*, Richard C; Giglione, Carmela; Meinnel*, Thierry "The Proteomics of N-Terminal Methionine Cleavage" Mol. Cell. Proteomics, 2006, 5, 2336-2349.
Copik, Alicja J.; Nocek, Boguslaw; Swierczek, Sabina I.; Ruebush, Shane; SeBok, Jang; D'souza, Ventris M.; Peters*, John; Bennett, Brian*; Holz*, Richard C "EPR and X-ray Crystallographic Characterization of the Product Bound Form of the Mn(II)-Loaded Methionyl Aminopeptidase from Pyrococcus furiosus" Biochemistry, 2005, 44, 121-129.
D'souza, Ventris M.; Brown, Robert S.; Bennett*, Brian; Holz, * Richard C. "Charaterization of the Active site and Insight into the Binding Mode of the Anti-Angiogenesis Agent Fumagillin to the Mn(II)-Loaded Methionyl Aminopeptidase from Escherichia coli" J. Biol. Inorg. Chem., 2005, 10, 41-50.
Copik, Alicja J.; Watterson, Sarah; Swierczek, Sabina I.; Bennett*, Brian; Holz*, Richard C. "Both Nucleophile and Substrate Bind to the Catalytic Fe(II) Center in the Type-II Methionyl Aminopeptidase from Pyrococcus furiosus" Inorg. Chem., 2005, 44, 1160-1162.
Rozhok, Sergey; Kwang-Fu Shen, Clifton; Littler, Pey-Lih Ho; Fan, Zhifang; Liu, Chang; Mirkin*, Chad A.; Holz*, Richard C. "Methods for Fabricating Microarrays of Motile Bacteria" Small, 2005, 1, 445-451.
Rozhok, Sergey; Holz*, Richard C. "Electrochemical Adhesion of Motile Bacteria to Gold" Tantala, 2005, 66, 538-542.
Swierczek, Krzysztof; Copik, Alicja J.; Swierczek, Sabina I.; Holz*, Richard C. "Molecular Discrimination of ype-I Methionyl Aminopeptidases Over Type-II" Biochemistry, 2005, 44, 12049-12056.
Bzymek, Krzysztof P.; Moulin, Aaron; Swierczek, Sabina I.; Bennett*, Brian; Ringe*, Dagmar; Petsko*, Gregory; Holz*, Richard C. "Kinetic, Spectroscopic, and X-ray Crystallographic Characterization of the Functional E151H Aminopeptidase from Aeromonas Proteolytica" Biochemistry, 2005, 44, 12030-12040.
McGregor, Wade; Swierczek, Sabina I.; Bennett, Brian*; Holz*, Richard C. "The argE -Encoded N -Acetyl-LOrnithine Deacetylase from Escherichia coli Contains a Dinuclear Metallo-Active Site" J. Am. Chem. Soc., 2005, 127, 14100-14107.
Bzymek, Krzysztof P.; Swierczek, Sabina I.; Bennett*, Brian; Holz*, Richard C. "Spectroscopic Characterization of the E151D and E151A Altered Aminopeptidases from Aeromonas Proteolytica" Inorg.Chem., 2005, 44, 8574-8580.

CURRENT FUNDING

National Science Foundation - (CHE-0652981) 03/1/06 -02/28/09 "Mechanistic Studies on Dinuclear Metallohydrolases that are Novel Antibacterial Targets" The long range goals of this research are to elucidate the roles of each metal ion is catalysis for the aminopeptidase from Aeromonas proteolytica and the dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase from Haemophilus influenzae (dapE). Using a mechanism based approach a new class of antibiotics that target dapE will be designed and synthesized.

Holz, Richard

Personal Information

NIH Postdoctoral Research Fellow, University of Minnesota, 1989 to 1992.

Ph.D. The Pennsylvania State University, Chemistry, 1989.

M.S. University of Minnesota-Duluth, Chemistry, 1986.

B.S. Bemidji State University, Major: Chemistry, Minors: Biology and Mathematics, 1984.

Invited speaker at the International Conference on Bioinorganic Chemistry, July 2009

Invited speaker at the 11th Nano Science and Technology Institute (NSTI)-Nanotech-08 Conference June, 2008

Invited speaker at the 235th American Chemical Society National Meeting, March 2008

Reviewer for the Center for Research and Education on Environmental Implications of Nanotechnology Program, NSF, Jan. 2008

Reviewer for the Collaborative Research in Chemistry Program, NSF, Nov. 2006

Reviewer for the Chemical Instrumentation Program, NSF, April 2005

Symposium Chair/Organizer, 59th Northwest Regional American Chemical Society Meeting, June 2004.

Institute for Nanotechnology and Department of Chemistry Visiting Research Professor, Northwestern University, 2002-03

Invited speaker at the Inaugural Gordon Research Conference on Metals in Medicine, July 2002

Ad-hoc Reviewer for Physical Biochemistry Study Section, NIH, March 2002

Symposium Chair/Organizer, Metals in Medicine 222nd ACS National Meeting, August 2001

Utah State University, College of Science Researcher of the Year, 2001

Board of Directors, Stokes Nature Center, Logan UT, 2001 to 2004

Ad-hoc Reviewer for the Physical Biochemistry Study Section, NIH, June 2000

Reviewer for the Chemical Instrumentation Program, NSF, May 2000

Invited speaker at the “International Conference on Bioinorganic Chemistry”, July 1999

Ad-hoc Reviewer for the Physical Biochemistry Study Section, NIH, June 1999

Eccles Foundation Research Award, 1998

Invited speaker at the Gordon Research Conference on Metals in Biology, January 1998

Eccles Foundation Research Award, 1996

Invited speaker at the 51st American Chemical Society Northwest Regional Meeting, June 1996

Invited speaker at the 209th American Chemical Society National Meeting, April 1995

National Institutes of Health Postdoctoral Research Fellow 1990-92

Mitra, Sanghamitra; Bennett*, Brian; Holz*, Richard C. "Mutation of H63 and its Catalytic Affect on the Methionine Aminopeptidase from Escherichia coli" Biochim. Biophys. Acta, 2009, 1794, 137-143.

Mitra, Sanghamitra; Job, Kathleen, M; Bennett*, Brian; Holz*, Richard C "Dissecting the Catalytic Role of D97 in the Methionyl Aminopeptidase from Escherichia coli" 6248-6259.

Rao, Saroja; Holz*, Richard C. "Analyzing the Catalytic Mechanism of the Fe-Type Nitrile Hydratase from Comamonas testosteroni Ni1" Biochemistry, 2008, 47, 12057–12064.

Waterson, Sarah; Mitra, Sanghamitra; Swierczek, Sabina I.; Meng, Lu; Holz*, Richard C. "Kinetic and Spectroscopic Analysis of the Catalytic Role of H79 in the Methionyl Aminopeptidase from Escherichia coli" Biochemistry, 2008, 47, 11885-11893.

Munih, Petra; Moulin, Aaron; Stamper, Carin; Bennett, Brian; Holz*, Richard C.; Ringe*, Dagmar; Petsko*, Gregory "X-ray Crystallographic Characterization of the Co(II)-substituted Tris-bound forms of the Aminopeptidase from Aeromonas proteolytica" J. Inorg. Biochem., 2007, 101, 1099-1107.

Nieman, J. Karl C.; Holz, Richard C.; Sims, Ronald C. "13C NMR Analysis of Biologically Produced Pyrene Residues By Mycobacterium Sp. KMS in the Presence of Humic Acid" Environ. Sci. Technol., 2007, 41, 242-249.

Mitra, Sanghamitra; Holz*, Richard C. "Unraveling the Catalytic Mechanism of Nitrile Hydratases" J. Biol. Chem., 2007, 282, 7397-7404.

McGregor, Wade; Bennett, Brian; Holz*, Richard C. "Kinetic and Spectroscopic Characterization of the Manganese(II)-Loaded argE-Encoded N-Acetyl-L-Ornithine Deacetylase from Escherichia coli" J. Biol. Inorg. Chem., 2007, 12, 603-613.

Desmarais, William; Bienvenue, L. David; Bzymek, Krzysztof P.; Holz*, Richard C.; Petsko*, A, Gregory; Ringe*, Dagmar "The High Resolution and Low pH Crystal Structures of the Aminopeptidase from Aeromonas proteolytica" J. Biol. Inorg. Chem., 2006, 11, 398-408.

Mitra, Sanghamitra; Jiracek, Jiri; Zertova, Miroslava; Zakova, Lenka;Holz*, Richard C "A New Colorometric Assay for Methionyl Aminopeptidases: Examination of the Binding of a New Class of Pseudopeptide Analog Inhibitors" Anal. Biochem., 2006, 357, 43-49.

Rozhok, Sergey; Fan, Zhifang; Nyamjav, Dorjderem; Liu, Chang; Mirkin, Chad A.; Holz*, Richard C. "Attachment of Motile Bacterial Cells in a Pre-Aligned Holed Microarrays" Langmuir., 2006, 22, 11251-11254.

Frottin, Frédéric; Martinez, Aude; Peynot, Philippe; Mitra, Sanghamitra; Holz*, Richard C; Giglione, Carmela; Meinnel*, Thierry "The Proteomics of N-Terminal Methionine Cleavage" Mol. Cell. Proteomics, 2006, 5, 2336-2349.

D'souza, Ventris M.; Brown, Robert S.; Bennett*, Brian; Holz, * Richard C. "Charaterization of the Active site and Insight into the Binding Mode of the Anti-Angiogenesis Agent Fumagillin to the Mn(II)-Loaded Methionyl Aminopeptidase from Escherichia coli" J. Biol. Inorg. Chem., 2005, 10, 41-50.

Copik, Alicja J.; Watterson, Sarah; Swierczek, Sabina I.; Bennett*, Brian; Holz*, Richard C. "Both Nucleophile and Substrate Bind to the Catalytic Fe(II) Center in the Type-II Methionyl Aminopeptidase from Pyrococcus furiosus" Inorg. Chem., 2005, 44, 1160-1162.

Rozhok, Sergey; Kwang-Fu Shen, Clifton; Littler, Pey-Lih Ho; Fan, Zhifang; Liu, Chang; Mirkin*, Chad A.; Holz*, Richard C. "Methods for Fabricating Microarrays of Motile Bacteria" Small, 2005, 1, 445-451.

Rozhok, Sergey; Holz*, Richard C. "Electrochemical Adhesion of Motile Bacteria to Gold" Tantala, 2005, 66, 538-542.

Swierczek, Krzysztof; Copik, Alicja J.; Swierczek, Sabina I.; Holz*, Richard C. "Molecular Discrimination of ype-I Methionyl Aminopeptidases Over Type-II" Biochemistry, 2005, 44, 12049-12056.

McGregor, Wade; Swierczek, Sabina I.; Bennett, Brian*; Holz*, Richard C. "The argE-Encoded N-Acetyl-LOrnithine Deacetylase from Escherichia coli Contains a Dinuclear Metallo-Active Site" J. Am. Chem. Soc., 2005, 127, 14100-14107.

Bzymek, Krzysztof P.; Swierczek, Sabina I.; Bennett*, Brian; Holz*, Richard C. "Spectroscopic Characterization of the E151D and E151A Altered Aminopeptidases from Aeromonas Proteolytica" Inorg.Chem., 2005, 44, 8574-8580.

Loyola

Invited speaker at the 11^th Nano Science and Technology Institute (NSTI)-Nanotech-08 Conference June, 2008

Symposium Chair/Organizer, 59^th Northwest Regional American Chemical Society Meeting, June 2004.

Symposium Chair/Organizer, Metals in Medicine 222^nd ACS National Meeting, August 2001

Mitra, Sanghamitra; Bennett, Brian; Holz, Richard C. "Mutation of H63 and its Catalytic Affect on the Methionine Aminopeptidase from Escherichia coli" Biochim. Biophys. Acta, 2009, 1794, 137-143.

Mitra, Sanghamitra; Job, Kathleen, M; Bennett, Brian; Holz, Richard C "Dissecting the Catalytic Role of D97 in the Methionyl Aminopeptidase from Escherichia coli" 6248-6259.

Rao, Saroja; Holz, Richard C. "Analyzing the Catalytic Mechanism of the Fe-Type Nitrile Hydratase from Comamonas testosteroni* Ni1" Biochemistry, 2008, 47, 12057–12064.

Waterson, Sarah; Mitra, Sanghamitra; Swierczek, Sabina I.; Meng, Lu; Holz, Richard C. "Kinetic and Spectroscopic Analysis of the Catalytic Role of H79 in the Methionyl Aminopeptidase from Escherichia coli*" Biochemistry, 2008, 47, 11885-11893.

Munih, Petra; Moulin, Aaron; Stamper, Carin; Bennett, Brian; Holz, Richard C.; Ringe, Dagmar; Petsko, Gregory "X-ray Crystallographic Characterization of the Co(II)-substituted Tris-bound forms of the Aminopeptidase from Aeromonas proteolytica*" J. Inorg. Biochem., 2007, 101, 1099-1107.

McGregor, Wade; Bennett, Brian; Holz, Richard C. "Kinetic and Spectroscopic Characterization of the Manganese(II)-Loaded argE -Encoded N -Acetyl-L-Ornithine Deacetylase from Escherichia coli*" J. Biol. Inorg. Chem., 2007, 12, 603-613.

Desmarais, William; Bienvenue, L. David; Bzymek, Krzysztof P.; Holz, Richard C.; Petsko, A, Gregory; Ringe, Dagmar "The High Resolution and Low pH Crystal Structures of the Aminopeptidase from Aeromonas proteolytica*" J. Biol. Inorg. Chem., 2006, 11, 398-408.

Frottin, Frédéric; Martinez, Aude; Peynot, Philippe; Mitra, Sanghamitra; Holz, Richard C; Giglione, Carmela; Meinnel, Thierry "The Proteomics of N-Terminal Methionine Cleavage" Mol. Cell. Proteomics, 2006, 5, 2336-2349.

D'souza, Ventris M.; Brown, Robert S.; Bennett, Brian; Holz, Richard C. "Charaterization of the Active site and Insight into the Binding Mode of the Anti-Angiogenesis Agent Fumagillin to the Mn(II)-Loaded Methionyl Aminopeptidase from Escherichia coli" J. Biol. Inorg. Chem., 2005, 10, 41-50.

Copik, Alicja J.; Watterson, Sarah; Swierczek, Sabina I.; Bennett, Brian; Holz, Richard C. "Both Nucleophile and Substrate Bind to the Catalytic Fe(II) Center in the Type-II Methionyl Aminopeptidase from Pyrococcus furiosus" Inorg. Chem., 2005, 44, 1160-1162.

Rozhok, Sergey; Kwang-Fu Shen, Clifton; Littler, Pey-Lih Ho; Fan, Zhifang; Liu, Chang; Mirkin, Chad A.; Holz, Richard C. "Methods for Fabricating Microarrays of Motile Bacteria" Small, 2005, 1, 445-451.

McGregor, Wade; Swierczek, Sabina I.; Bennett, Brian; Holz, Richard C. "The argE -Encoded N -Acetyl-LOrnithine Deacetylase from Escherichia coli Contains a Dinuclear Metallo-Active Site" J. Am. Chem. Soc., 2005, 127, 14100-14107.

Bzymek, Krzysztof P.; Swierczek, Sabina I.; Bennett, Brian; Holz, Richard C. "Spectroscopic Characterization of the E151D and E151A Altered Aminopeptidases from Aeromonas Proteolytica" Inorg.Chem., 2005, 44, 8574-8580.